Journal: Experimental and Therapeutic Medicine

Article Title: Effects of norepinephrine on colonic tight junction protein expression during heat stress

doi: 10.3892/etm.2021.9865

Figure Lengend Snippet: Expression levels of Occludin, ZO-1, TRPA1 and PAR-2 in the rat distal colon tissue sections in control and heat exposure groups. The figures (A, B) shown are representative of three different animals. Magnification, x200. Black arrows indicate the musca. Red arrows indicate the myenteric plexus. ZO-1, zonula occludens-1; PAR-2, protease-activated receptor 2; TRPA1, transient receptor potential ankyrin 1 channel; CM, circular muscle; LM, longitudinal muscle.

Article Snippet: The cells were then incubated with primary antibodies against PAR-2 (1:300; cat. no. bs-1178R; BIOSS), Occludin (1:100; cat. no. ab216327; Abcam), ZO-1 (1:300; cat. no. bs-1329-R; BIOSS) and TRPA1 (1:200; cat. no. ab58844; Abcam) at 4˚C overnight, followed by incubation with FITC-conjugated goat-anti-rabbit secondary antibodies (1:50; cat. no. bs-0295G-FITC; BIOSS) for 1 h at room temperature in the dark after washing in cold PBS.

Techniques: Expressing

Journal: Experimental and Therapeutic Medicine

Article Title: Effects of norepinephrine on colonic tight junction protein expression during heat stress

doi: 10.3892/etm.2021.9865

Figure Lengend Snippet: Expression levels of Occludin, ZO-1, PAR-2 and TRPA1 in Caco-2 cells after treatment with NE. (A) Representative western blotting images from four independent experiments. Comparison of the relative expression levels of (B) Occludin, (C) ZO-1, (D) PAR-2 and (E) TRPA1 in colon of control and NE-treated cells. * P<0.05 vs. Control. Data are presented as the mean ± SD. NE, norepinephrine; ZO-1, zonula occludens-1; PAR-2, protease activated receptor 2; TRPA1, transient receptor potential ankyrin 1 channel.

Article Snippet: The cells were then incubated with primary antibodies against PAR-2 (1:300; cat. no. bs-1178R; BIOSS), Occludin (1:100; cat. no. ab216327; Abcam), ZO-1 (1:300; cat. no. bs-1329-R; BIOSS) and TRPA1 (1:200; cat. no. ab58844; Abcam) at 4˚C overnight, followed by incubation with FITC-conjugated goat-anti-rabbit secondary antibodies (1:50; cat. no. bs-0295G-FITC; BIOSS) for 1 h at room temperature in the dark after washing in cold PBS.

Techniques: Expressing, Western Blot

Journal: Experimental and Therapeutic Medicine

Article Title: Effects of norepinephrine on colonic tight junction protein expression during heat stress

doi: 10.3892/etm.2021.9865

Figure Lengend Snippet: Immunofluorescence staining of Occludin, ZO-1, PAR-2 and TRPA1 in Caco-2 cells after 10 µM NE application. Staining of (A) Occludin, (B) ZO-1, (C) PAR-2 and (D) TRPA1. Magnification, x400. NE, norepinephrine; ZO-1, zonula occludens-1; PAR-2, protease-activated receptor 2; TRPA1, transient receptor potential ankyrin 1 channel.

Article Snippet: The cells were then incubated with primary antibodies against PAR-2 (1:300; cat. no. bs-1178R; BIOSS), Occludin (1:100; cat. no. ab216327; Abcam), ZO-1 (1:300; cat. no. bs-1329-R; BIOSS) and TRPA1 (1:200; cat. no. ab58844; Abcam) at 4˚C overnight, followed by incubation with FITC-conjugated goat-anti-rabbit secondary antibodies (1:50; cat. no. bs-0295G-FITC; BIOSS) for 1 h at room temperature in the dark after washing in cold PBS.

Techniques: Immunofluorescence, Staining